Integrative strategy to determine residual proteins in cefaclor produced by immobilized penicillin G acylase
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چکیده
منابع مشابه
Enzymatic Synthesis of Amoxicillin with Immobilized Penicillin G Acylase
The synthesis of amoxicillin with immobilized penicillin G acylase (PGA) in aqueous medium was investigated. The parameters studied were: time course of amoxicillin production, concentration of substrates: hydroxyphenylglycine methyl ester (HPGM) and 6-aminopeicillanic acid (6APA) and the e ect of enzyme (PGA) content and pH, under variable and constant conditions and temperature variations. In...
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Ye-Wang Zhang, Shu-Lai Liu, Dong-Zhi Wei, Rui-Jiang Liu, and Xi-Ming Xu School of Pharmaceutics, Jiangsu University, Zhenjiang, 212013, P. R. China State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, P. R. China College of Biological and Environmental Engineering, Zhejiang University of Technolo...
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Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized penicillin G acylase biocatalysts of average particle size of 5·10m and 10·10m at protein loadin...
متن کاملImproving Selectivity and Productivity of the Enzymatic Synthesis of Ampicillin with Immobilized Penicillin G Acylase
An experimental design was applied to improve the reaction conditions for enzymatic synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA), catalyzed by penicillin G acylase from E. coli immobilized on an agarose-glyoxyl derivative. The presence and magnitude of interactions between reaction variables were estimated using a 2 factorial design. A batc...
متن کاملThe realm of penicillin G acylase in -lactam antibiotics
Penicillin G acylase (PGA; EC 3.5.1.11) is a hydrolytic enzyme that acts on the side chains of penicillin G, cephalosporin G and related ntibiotics to produce the -lactam antibiotic intermediates 6-amino penicillanic acid (6-APA) and 7-amino des-acetoxy cephalosporanic acid 7-ADCA), with phenyl acetic acid (PAA) as a common by-product. These antibiotic intermediates are among the potential buil...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical and Biomedical Analysis
سال: 2020
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2020.113229